ELISA Recombinant Mesencephalic astrocyte-derived neurotrophic factor protein(MANF) (Active)

Quantity:100µg. Research Areas:Neuroscience Uniprot NO.:P55145 Uniprot Entry Name:MANF_ Gene Names:MANF,ARMET,ARP Species:Homo sapiens () Source:E.Coli Expression Region:25-182aa Sequence:LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL Protein Description:FµLl Length of Mature Protein Tag Info:Tag-Free Mol. Weight:18.2 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 ?g/mL, corresponding to a specific activity of ? 50 IU/mg. Purity:>95% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Arginine-rich protein, Relevance:Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. ModµLates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticµLum (ER) stress-induced cell death. {ECO:0000250, ECO:0000269|PubMed:12794311, ECO:0000269|PubMed:18561914}. PubMed ID:8649854; 15489334; 16641997; 12665801; 12794311; 15144186; 18561914; 21269460; 24275569; 19258449; 8971156; 9174057 Function:Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. ModµLates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticµLum (ER) stress-induced cell death. Involvement in disease: SubcellµLar Location:Secreted Protein Families:ARMET family Tissue Specificity: Paythway: HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:15461 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=436446 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:7873 STRING Database Link:https://string-db.org/network/9606.ENSP00000432799 OMIM Database Link:https://www.omim.org/entry/601916601916601916